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Bachem
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Image Search Results
Journal: bioRxiv
Article Title: Ghrelin signaling regulates feeding behavior, metabolism, and memory through the vagus nerve
doi: 10.1101/2020.06.16.155762
Figure Lengend Snippet: The left and right nodose ganglia (LNG, RNG), which house VAN cell bodies, express Ghsr mRNA in both rats (A) and mice (B) with no differences in expression between LNG and RNG in either species. Ghsr mRNA (green) is exclusively expressed in rat nodose ganglion neurons (red, neuronal marker NeuN ), as demonstrated co-localization of Ghsr and NeuN (yellow), and approximately ∼73% of NeuN + nodose ganglion neurons co-express Ghsr (C). After complete bilateral subdiaphragmatic vagotomy in rats (D), the hyperphagic effect of IP ghrelin at 40μg/kg BW injected in the early dark cycle was abolished compared with controls (E). VAN Ghsr expression is knocked down in rats via bilateral nodose ganglia injections of a custom-designed AAV (AAV-2 GFP-rGHSR-shRNA) (F). Representative histology of VAN cell bodies in the nodose ganglion expressing the GHSR shRNA AAV with a green fluorescent protein (GFP) transgene (G). Gene expression analyses confirmed a statistically significant ∼86% knockdown of the Ghsr gene in the nodose ganglion (H). All data presented as mean +/- SEM.
Article Snippet: IP
Techniques: Expressing, Marker, Injection, shRNA, Gene Expression, Knockdown